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prsfduet-1

pacycduet

petduet-1

pcdf 1b plasmid

cdf origin of replication

clodf13 origin

pcoladuet-1

pcdfduet-1 vector




pCDFDuet™-1. Bacterial vector with a CloDF13 (CDF) origin for the co-expression of two genes. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.
pCDFDuet™-1 DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS; CoA; User Protocol; Citations; Vector Map; Vector Sequence. 71340: Price could not be retrieved: Minimum Quantity needs to be mulitiple of: Upon Order Completion More
The pET-43.1 and pET-44 vectors are sold under patent license from University of Oklahoma for research use only. Licenses for Vectors containing the His•Tag® sequence are licensed under U. S. Patent Nos. 5,310,663 .. The pET, pRSF-2, and pCDF-2 Ek/LIC vectors are compatible with each other in the same cell and.
pCDFDuet-1 Vector. pCDFDuet-1 sequence landmarks. T7 promoter-1. 3765–3781. T7 transcription start-1. 1. His•Tag® coding sequence. 83–100. Multiple cloning sites-1. (Nco I–Afl II). 69–168. T7 promoter-2. 214–230. T7 transcription start-2. 231. Multiple cloning sites-2. (Nde I–Avr II). 297–438. S•Tag™ coding sequence.
pCDF cDNA Expression Lentivectors. Cat. #s CD100A-1 – CD111B-1. C. Map and Features for pCDF1-MCS2-EF1-copGFP Vector. Feature. Location*. Function. CMV/5'LTR. 1-415. Hybrid CMV promoter-R/U5 long terminal repeat; required for viral packaging and transcription gag. 762-1011. Packaging signal. RRE. 1012-
3' Sequencing 2 Primer Sequence: GCTAGTTATTGCTCAGCGG. 5' Terminal: N-Term. 5' Terminal 2: N-Term. 3' Terminal: C-Term. 3' Terminal 2: C-Term. Tag 1: 6xHis. Tag 2: S15. Bacterial Resistance: Streptomycin. Notes: pCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two
pCDF-1b carries an N-terminal His•Tag® coding sequence that is cleavable with enterokinase as well as an optional C-terminal S•Tag™ coding sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB392). Commercial use of this Product
cat gene (chloramphenicol resistance), and pCDFDuet-1 carries the CloDF13 replicon (12) and. aadA gene (streptomycin/spectinomycin resistance). Two kanamycin-resistant Duet vectors are available; pRSFDuet-1 carries the RSF1030 replicon (13, 14) and pCOLADuet-1 carries the ColA replicon (15). Each vector carries
User Manual. I. Introduction and Background. A. Purpose of this Manual. This manual provides details and information necessary to generate expression constructs of your gene of interest in the pCDF lentivectors. Specifically, it provides critical instructions on amplification and cloning the cDNA into the pCDF Vectors, and.
This plasmid carries an origin derived from CloDF13 (1) and streptomycin/spectinomycin resistance, allowing for the option of coexpression with many other Novagen T7-based expression vectors. The vector contains a T7 promoter, lac operator, ribosome binding site (rbs), an amino terminal His•Tag® coding sequence,
     

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